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Polyclonal antibodies against PSA and PSAP (both from Dako Corp ¬ ; 1:1800), and monoclonal antibody against AR (Novocastra ¬1:10) were used. Biotin-labeled secondary antibody and ABC complex (Vector ¬ ) followed by exposure to diaminobenzidine tetrahydrochloride (Sigma Corp ¬ ) were used to detect the antigen. The slides were counter-stained with hematoxylin and mounted in Permount.

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